Disabling USB drives in VISTA

You can do this via a group policy. First copy the data presented in this article from microsoft into a .adm. The data you need to paste is also presented below:

CLASS MACHINE
CATEGORY !!category
CATEGORY !!categoryname
POLICY !!policynameusb
KEYNAME "SYSTEM\CurrentControlSet\Services\USBSTOR"
EXPLAIN !!explaintextusb
PART !!labeltextusb DROPDOWNLIST REQUIRED

VALUENAME "Start"
ITEMLIST
NAME !!Disabled VALUE NUMERIC 3 DEFAULT
NAME !!Enabled VALUE NUMERIC 4
END ITEMLIST
END PART
END POLICY
POLICY !!policynamecd
KEYNAME "SYSTEM\CurrentControlSet\Services\Cdrom"
EXPLAIN !!explaintextcd
PART !!labeltextcd DROPDOWNLIST REQUIRED

VALUENAME "Start"
ITEMLIST
NAME !!Disabled VALUE NUMERIC 1 DEFAULT
NAME !!Enabled VALUE NUMERIC 4
END ITEMLIST
END PART
END POLICY
POLICY !!policynameflpy
KEYNAME "SYSTEM\CurrentControlSet\Services\Flpydisk"
EXPLAIN !!explaintextflpy
PART !!labeltextflpy DROPDOWNLIST REQUIRED

VALUENAME "Start"
ITEMLIST
NAME !!Disabled VALUE NUMERIC 3 DEFAULT
NAME !!Enabled VALUE NUMERIC 4
END ITEMLIST
END PART
END POLICY
POLICY !!policynamels120
KEYNAME "SYSTEM\CurrentControlSet\Services\Sfloppy"
EXPLAIN !!explaintextls120
PART !!labeltextls120 DROPDOWNLIST REQUIRED

VALUENAME "Start"
ITEMLIST
NAME !!Disabled VALUE NUMERIC 3 DEFAULT
NAME !!Enabled VALUE NUMERIC 4
END ITEMLIST
END PART
END POLICY
END CATEGORY
END CATEGORY

[strings]
category="Custom Policy Settings"
categoryname="Restrict Drives"
policynameusb="Disable USB"
policynamecd="Disable CD-ROM"
policynameflpy="Disable Floppy"
policynamels120="Disable High Capacity Floppy"
explaintextusb="Disables the computers USB ports by disabling the usbstor.sys driver"
explaintextcd="Disables the computers CD-ROM Drive by disabling the cdrom.sys driver"
explaintextflpy="Disables the computers Floppy Drive by disabling the flpydisk.sys driver"
explaintextls120="Disables the computers High Capacity Floppy Drive by disabling the sfloppy.sys driver"
labeltextusb="Disable USB Ports"
labeltextcd="Disable CD-ROM Drive"
labeltextflpy="Disable Floppy Drive"
labeltextls120="Disable High Capacity Floppy Drive"
Enabled="Enabled"
Disabled="Disabled"

Run the group policy editor: "gpedit.msc"

Afterwards goto "Computer Configuration>Administrative Templates" since you want to disable it for the computer. Right click "Administrative Templates" and select "add / remove template". Click "Add" and browse to the .adm file you made. After adding the file close the dialog.

A new section "Classic Administrative Templates (ADM)" will appear. Go inside that folder and you will see "Custom Policy Settings>Restrict Drives". In case the Restrict Drives folder is empty you need to do the following: goto "View>Filtering" and uncheck "Only show policy settings that can be fully managed".

You will now see the option to "Disable USB Drives" along with others in the "Restrict Drives" folder. Click on it. Select Enabled and also "Disable USB Ports" to Enabled.